Anti-inflammatory effect of pristimerin on TNFα-induced inflammatory responses in murine macrophages

Purpose: To investigate the anti-inflammatory effect of pristimerin using RAW 264.7 macrophage cells. Methods: The effect of pristimerin on the RAW 264.7 cells proliferation was determined by CCK8 test. The expression of cyclin D1 and cyclin E1 in RAW 264.7 cells treated with TNF-α and pristimerin was measured by qRT-PCR. The regulation of cytokine IL-1β, IL-6, IL-8, and PGE2 in RAW 264.7 cells treated by pristimerin was measured by ELISA. Results: TNF-α can induce the proliferation of RAW 264.7 cells in a dose-dependent manner, co-treatment with pristimerin results in a significant suppression in the proliferation of RAW 264.7 cells. Besides, pristimerin suppressed the expression of cyclin D1 and cyclin E1 induced by TNF-α. TNF-α had a dose-dependent induction on the production of IL-1β, IL-6, IL-8, and PGE2. Pristimerin inhibited the production of IL-1β, IL-6, IL-8, and PGE2 in RAW 264.7 cells. TNF-α increased the production of NO and iNOS in RAW 264.7 cells, which can be blocked by pristimerin. Conclusion: Pristimerin is an anti-inflammatory agent on macrophage, it realize this function through the following ways, first pristimerin reduce the production of pro-inflammatory cytokines; then pristimerin inhibit the expression of inflammatory mediators NO and iNOS; finally pristimerin inhibits the proliferation of RAW 264.7 cells through cell cycle arrest.